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polymorphonuclear neutrophils pmns  (Bio-Rad)


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    Bio-Rad polymorphonuclear neutrophils pmns
    Polymorphonuclear Neutrophils Pmns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 417 article reviews
    polymorphonuclear neutrophils pmns - by Bioz Stars, 2026-05
    96/100 stars

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    ( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
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    ( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with PMN, CD3, GFAP, NeuN, and GST-π. ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Journal: Science Advances

    Article Title: Macrophage centripetal migration drives spontaneous healing process after spinal cord injury

    doi: 10.1126/sciadv.aav5086

    Figure Lengend Snippet: ( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with PMN, CD3, GFAP, NeuN, and GST-π. ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Article Snippet: The following primary antibodies were used: CD68 (rat, MCA1957; 1:200; Serotec), GFAP (rabbit, Z0334; 1:200; Dako), IRF8 (goat, sc-6058; 1:200; Santa Cruz Biotechnology), GST-π (mouse, no. 610719; 1:200; BD Biosciences), NeuN (mouse, MAB377; 1:200; Chemicon), PMN (rat, MCA771GA; 1:200; Serotec), CD3 (145-2C11; 1:200; eBioscience), 5-HT (goat, no.20079; 1:200; ImmunoStar), platelet-derived growth factor receptor-β (PDGFRβ) (rat, no.136002; 1:200; BioLegend), and C5a (rabbit, BS-0324R; 1:200; Bioss).

    Techniques: Staining, Expressing, Fluorescence, Double Immunostaining, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Isolation